Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ryan, M.; Liu, T.; Dahlquist, F.W.; Griffith, O.H.
    A catalytic diad involved in substrate-assisted catalysis: NMR study of hydrogen bonding and dynamics at the active site of phosphatidylinositol-specific phospholipase C (2001), Biochemistry, 40, 9743-9750.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.6.1.13
-
 Bacillus cereus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.6.1.13 X-ray crystal structure Bacillus cereus

Protein Variants

EC Number Protein Variants Comment Organism
4.6.1.13 D274A mutation of an catalytic diad residue, mutant with abolished activity, NMR study Bacillus cereus
4.6.1.13 D274N mutation of an catalytic diad residue, 4.2% of wild-type activity Bacillus cereus
4.6.1.13 H32A mutation of an catalytic diad residue, mutant with abolished activity, NMR study Bacillus cereus

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.13 Bacillus cereus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.13 recombinant wild-type and mutant PI-PLC Bacillus cereus

Reaction

EC Number Reaction Comment Organism Reaction ID
4.6.1.13 1-phosphatidyl-1D-myo-inositol = 1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol mechanism Bacillus cereus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.13 1-phosphatidyl-1D-myo-inositol a catalytic diad at the active site composed of Asp-274 and His-32 is involved in substrate-assisted catalysis, its function is to hydrogen-bond with the 2-OH of phosphatidylinositol to form a catalytic triad, catalytic mechanism Bacillus cereus 1D-myo-inositol 1,2-cyclic phosphate + diacylglycerol PI-PLC catalyzes in a second step the slow hydrolysis of 1D-myo-inositol 1,2-cyclic phosphate to form myo-inositol 1-phosphate ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.6.1.13 37
-
 assay at Bacillus cereus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.6.1.13 7
-
 assay at Bacillus cereus